7XEX
Crystal strucutre of apoCasDinG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 264.828, 72.232, 139.428 |
| Unit cell angles | 90.00, 121.22, 90.00 |
Refinement procedure
| Resolution | 38.370 - 3.000 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fwr |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 44576 | 4125 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.1 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.845 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 8% PEG 6000, 0.1M HEPES 7.4 |
