7XE7

T4 lysozyme mutant-S44C/C54T/N68C/A93C/C97A/T115C, pH10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-25
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	59.976, 59.976, 97.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.970 - 1.050
R-factor	0.1645
Rwork	0.164
R-free	0.17780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vnr
RMSD bond length	0.005
RMSD bond angle	0.968
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	1.090
High resolution limit [Å]	1.050	1.050
Rmeas	0.054	0.401
Number of reflections	93665	8688
<I/σ(I)>	27	3.1
Completeness [%]	98.8	92.5
Redundancy	10	6.4
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	A crystal was soaked into precipitant-free solutions (pH10)