7XDX

Crystal structure of a receptor like kinase from Arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	61.670, 82.950, 61.940
Unit cell angles	90.00, 93.30, 90.00

Refinement procedure

Resolution	61.568 - 2.230
R-factor	0.1843
Rwork	0.181
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qkw
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.950	2.290
High resolution limit [Å]	2.230	2.230
Rmerge	0.111
Number of reflections	30350	28857
<I/σ(I)>	9
Completeness [%]	99.5
Redundancy	3.2
CC(1/2)		0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Tris pH 8.0, 30% v/v Polyethylene glycol 400