7XDW

Crystal structure of a receptor like kinase from Arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	61.805, 83.163, 62.048
Unit cell angles	90.00, 93.14, 90.00

Refinement procedure

Resolution	61.712 - 1.926
R-factor	0.1877
Rwork	0.187
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qkw
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.950	2.030
High resolution limit [Å]	1.926	1.930
Rmerge	0.057
Number of reflections	42862	42809
<I/σ(I)>	13
Completeness [%]	90.2
Redundancy	3.3
CC(1/2)		0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Potassium chloride, 0.01M Magnesium chloride hexahydrate, 0.05M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400