7XDV

Crystal structure of a receptor like kinase from Arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	61.950, 83.330, 62.050
Unit cell angles	90.00, 93.39, 90.00

Refinement procedure

Resolution	27.059 - 1.970
R-factor	0.1723
Rwork	0.171
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qkw
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.760	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.086
Number of reflections	44514	44474
<I/σ(I)>	12.1
Completeness [%]	99.8
Redundancy	6.7
CC(1/2)		0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.05M Magnesium chloride hexahydrate, 0.1M HEPES 7.5, 30% v/vPolyethylene glycol monomethyl ether 550