7XCB

Crystal structure of the mouse interleukin-9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	95.500, 95.500, 105.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.570 - 3.400
R-factor	0.2436
Rwork	0.241
R-free	0.29030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-P15247-F1
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.490
High resolution limit [Å]	3.400	3.400
Rmeas	0.062	0.228
Number of reflections	4255	297
<I/σ(I)>	41.13
Completeness [%]	99.7	100
Redundancy	19.82	18.55
CC(1/2)	0.850	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	277.15	0.1M citric acid(pH 3.5), 2.0M Ammonium sulfate