7XBR
Crystal structure of phosphorylated AtMKK5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.666, 198.572, 202.824 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.130 - 3.200 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bf2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.670 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.167 | 1.697 |
Number of reflections | 55113 | 5452 |
<I/σ(I)> | 15.6 | 1.86 |
Completeness [%] | 96.7 | 97.2 |
Redundancy | 7.5 | 7.6 |
CC(1/2) | 0.990 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M Bis-Tris Propane (pH 7.0), 2.8 M NaAc (pH 7.0) and 0.4M NaCl |