7XBL

Dimeric structure of human galectin-7 in complex with three glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	52.851, 65.009, 73.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.930 - 2.000
R-factor	0.1741
Rwork	0.169
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.930	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.044	0.064
Number of reflections	17567	1255
<I/σ(I)>	31.2
Completeness [%]	99.5
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Hepes pH7.5, 12% PEG 3350