7XBH
The complex structure of RshSTT182/200 RBD bound to human ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-21 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.979183 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.413, 155.229, 60.996 |
Unit cell angles | 90.00, 103.66, 90.00 |
Refinement procedure
Resolution | 29.640 - 3.020 |
R-factor | 0.2023 |
Rwork | 0.201 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lzg |
RMSD bond length | 0.003 |
RMSD bond angle | 0.589 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19rc3_4028) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.420 | 2.920 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.166 | 2.842 |
Number of reflections | 37596 | 2783 |
<I/σ(I)> | 9.4 | 0.9 |
Completeness [%] | 99.8 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.01M sodium borate pH 8.5, 1.0M sodium citrate |