7XBF
The complex structure of RshSTT182/200 RBD-insert2 bound to human ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-25 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.391, 123.891, 112.787 |
Unit cell angles | 90.00, 93.26, 90.00 |
Refinement procedure
Resolution | 40.630 - 3.510 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lzg |
RMSD bond length | 0.002 |
RMSD bond angle | 0.498 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.650 |
High resolution limit [Å] | 3.510 | 3.510 |
Rmerge | 0.133 | 1.060 |
Number of reflections | 28025 | 2812 |
<I/σ(I)> | 9 | 1.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M MES pH6.0, 0.2M magnesium chloride, 20% w/v PEG 6000 |