7XAY

Crystal structure of Hat1-Hat2-Asf1-H3-H4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-10-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	96.410, 124.684, 161.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.400 - 3.300
R-factor	0.2321
Rwork	0.230
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4psw
RMSD bond length	0.003
RMSD bond angle	0.595
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.590	3.500
High resolution limit [Å]	3.300	3.300
Number of reflections	29577	4740
<I/σ(I)>	9.4	3
Completeness [%]	99.0
Redundancy	6.6
CC(1/2)	0.993	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	100 mM Bis-Tris propane, pH 6.5, 20% PEG-3350, and 200 mM sodium nitrate