7XAN
Structure of a triple-helix region of human collagen type III from Trautec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 15.783, 19.439, 83.164 |
| Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
| Resolution | 20.755 - 1.500 |
| Rwork | 0.167 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cuo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.694 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.490 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.021 | 0.058 |
| Number of reflections | 8076 | 360 |
| <I/σ(I)> | 48.1 | |
| Completeness [%] | 97.4 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.04M K phosphate monobasic, 16% w/v PEG 8000, 20% v/v Glycerol |
