7X9Z

Thermotoga maritima ferritin variant-Tm-E(G40E) with Co

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.597735
Spacegroup name	P 21 21 21
Unit cell lengths	54.517, 65.809, 91.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.340 - 2.290
R-factor	0.1939
Rwork	0.188
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	DIALS
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.340	2.372
High resolution limit [Å]	2.290	2.290
Number of reflections	29843	2470
<I/σ(I)>	13.89
Completeness [%]	98.6
Redundancy	2
CC(1/2)	0.991	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25mM HEPES, pH 8.0, 55% MPD, 5mM Cobalt chloride