7X9X

Thermotoga Maritima ferritin variant-Tm-E(G40E) with Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.276911
Spacegroup name	P 21 21 21
Unit cell lengths	55.500, 66.620, 92.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.560 - 2.300
R-factor	0.1957
Rwork	0.190
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.560	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	31502	3042
<I/σ(I)>	5.9
Completeness [%]	99.7
Redundancy	1.9
CC(1/2)	0.997	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25mM HEPES pH 8.0, 35% MPD, 2mM Zinc chloride