7X99
Anabolic ornithine carbamoyltransferases (OTCs) from Psychrobacter sp. PAMC 21119
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 |
| Unit cell lengths | 129.610, 129.610, 75.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.760 - 2.200 |
| Rwork | 0.183 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ort |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.596 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.760 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 1.326 | |
| Number of reflections | 36629 | 3171 |
| <I/σ(I)> | 25.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20.9 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Sodium Acetate: HCl, pH 4.6, 1M Ammonium Citrate Dibasic |
