7X8G

Crystal structure of ENL T1(H116P) mutant YEATS domain in complex with histone H3 acetylation at K27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-13
Detector	MAR CCD 130 mm
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	155.143, 48.501, 47.791
Unit cell angles	90.00, 101.13, 90.00

Refinement procedure

Resolution	27.100 - 1.910
R-factor	0.1983
Rwork	0.197
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.124	0.096	0.423
Rmeas	0.135	0.105	0.461
Rpim	0.053	0.042	0.180
Total number of observations	179110
Number of reflections	27427	1432	1381
<I/σ(I)>	4.7
Completeness [%]	100.0	99.6	100
Redundancy	6.5	6.3	6.4
CC(1/2)		0.988	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	20 % (w/v) PEG 4000, 0.1M sodium citrate pH 5.6, 20 % (v/v) 2-propanol