7X8F

Crystal structure of ENL T4 mutant YEATS domain in complex with histone H3 acetylation at K27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-06
Detector	MAR CCD 130 mm
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	156.556, 48.592, 47.259
Unit cell angles	90.00, 101.16, 90.00

Refinement procedure

Resolution	38.400 - 2.440
R-factor	0.2044
Rwork	0.202
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.490
High resolution limit [Å]	2.440	6.640	2.450
Rmerge	0.149	0.081	0.931
Rmeas	0.163	0.088	1.043
Rpim	0.063	0.035	0.461
Number of reflections	13211	701	662
<I/σ(I)>	3.7
Completeness [%]	100.0	99.9	100
Redundancy	6.4	6.1	5
CC(1/2)		0.992	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	20 % (w/v) PEG 4000, 0.1 M sodium citrate pH 5.6, 20 % (v/v) 2-propanol