7X8B

Crystal structure of ENL T1 mutant YEATS domain in complex with histone H3 acetylation at K27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-10
Detector	MAR CCD 130 mm
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	156.732, 48.670, 47.654
Unit cell angles	90.00, 101.17, 90.00

Refinement procedure

Resolution	17.330 - 2.300
R-factor	0.1898
Rwork	0.188
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.340
High resolution limit [Å]	2.300	6.230	2.300
Rmerge	0.091	0.053	0.333
Rmeas	0.102	0.060	0.368
Rpim	0.045	0.027	0.155
Total number of observations	80119
Number of reflections	15862	799	801
<I/σ(I)>	15.5
Completeness [%]	99.6	95.3	100
Redundancy	5.1	4.6	5.5
CC(1/2)		0.997	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20 % (w/v) PEG 4000, 0.1M sodium citrate pH5.6, 20 % (v/v) 2-propanol