7X85

Crystal structure of chicken CENP-C Cupin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.99999
Spacegroup name	P 61 2 2
Unit cell lengths	112.587, 112.587, 212.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.558 - 2.639
Rwork	0.185
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpv
RMSD bond length	0.006
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BUCCANEER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.750	48.750	2.770
High resolution limit [Å]	2.639	8.750	2.640
Rmerge	0.112	0.025	2.821
Rmeas	0.118	0.027	2.963
Rpim	0.036	0.009	0.902
Number of reflections	24156	790	3093
<I/σ(I)>	17.8
Completeness [%]	99.8
Redundancy	19.3	14.9	19.9
CC(1/2)	0.999	0.999	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M lithium citrate, 0.1 M MES-NaOH pH 6.5 and 20% PEG4000