7X7W

The X-ray Crystallographic Structure of D-Psicose 3-epimerase from Clostridia bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	I 1 2 1
Unit cell lengths	60.018, 70.657, 153.784
Unit cell angles	90.00, 99.32, 90.00

Refinement procedure

Resolution	37.939 - 2.097
R-factor	0.2029
Rwork	0.200
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vnk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.14_3260)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.950	2.160
High resolution limit [Å]	2.090	2.100
Rmerge	0.032	0.124
Number of reflections	37135	2994
<I/σ(I)>	30.4
Completeness [%]	99.4
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Succine acid pH 7.0, 0.1 M HEPES pH7.0, 1% w/v PEG MME 2000