7X7O

SARS-CoV-2 spike RBD in complex with neutralizing antibody UT28K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	I 1 2 1
Unit cell lengths	138.249, 138.412, 221.107
Unit cell angles	90.00, 90.05, 90.00

Refinement procedure

Resolution	48.140 - 3.750
R-factor	0.305
Rwork	0.303
R-free	0.33580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jmp
RMSD bond length	0.005
RMSD bond angle	0.836
Data reduction software	XDS (VERSION Feb 5, 202)
Data scaling software	Aimless (0.7.7)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.910	3.890
High resolution limit [Å]	3.750	3.750
Number of reflections	42875	16957
<I/σ(I)>	7.1
Completeness [%]	99.9
Redundancy	3.6
CC(1/2)	0.921	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Citric acid pH5.0, 1.0 M Lithium chloride, 10% (w/v) PEG 6000