7X5G
Nrf2 (A510Y)-MafG heterodimer bound with CsMBE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 246.022, 55.018, 86.152 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 33.890 - 2.300 |
| R-factor | 0.222 |
| Rwork | 0.219 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a5t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.454 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.037 | 0.520 |
| Number of reflections | 50434 | 2456 |
| <I/σ(I)> | 34.2 | 2.7 |
| Completeness [%] | 98.3 | 96.5 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 50 mM Tris-HCl (pH 7.5), 100 mM KCl, 10% polyethylene glycol monomethyl ether 550, 2% (v/v) glycerol |
