7X5E
Nrf2-MafG heterodimer bound with CsMBE1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 257.547, 54.840, 82.274 |
| Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
| Resolution | 40.850 - 2.300 |
| R-factor | 0.2149 |
| Rwork | 0.212 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a5t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.526 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.430 | |
| Number of reflections | 50732 | 5033 |
| <I/σ(I)> | 12.2 | 2.5 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 50 mM MES monohydrate (pH 5.6), 20 mM KCl, 10 mM MgSO4 heptahydrate, 7%(v/v) PEG400 |
