7X5D

Crystal Structure of the K316C mutant of Human Lamin A/C Coil 2 (residues 244-340)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	29.788, 40.966, 140.714
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.100 - 1.820
R-factor	0.2409
Rwork	0.239
R-free	0.27920
Structure solution method	AB INITIO PHASING
Data scaling software	HKL-2000
Phasing software	Arcimboldo
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.100	20.000	1.830
High resolution limit [Å]	1.800	4.860	1.800
Rmerge	0.062	0.050	0.334
Rmeas	0.066	0.053	0.355
Rpim	0.020	0.017	0.117
Number of reflections	15449	913	541
<I/σ(I)>	12
Completeness [%]	95.1	99.1	68.4
Redundancy	9.5	12	6.8
CC(1/2)		0.998	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	Tacsimate (pH 5.5), PEG 3350, methyl mercury acetate