7X4X

BTB domain of KEAP1 in complex with MEF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.58
Spacegroup name	P 21 21 21
Unit cell lengths	84.322, 99.289, 143.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.390 - 2.960
R-factor	0.2076
Rwork	0.203
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cxi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.390	3.100
High resolution limit [Å]	2.960	3.000
Number of reflections	25315	2191
<I/σ(I)>	8
Completeness [%]	98.1
Redundancy	5.1
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M lithium acetate, 20% PEG 3350