7X4V
Crystal structure of Rhodostomin ARGDDP mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.293, 62.092, 34.756 |
| Unit cell angles | 90.00, 105.61, 90.00 |
Refinement procedure
| Resolution | 18.090 - 1.380 |
| R-factor | 0.18469 |
| Rwork | 0.183 |
| R-free | 0.21689 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rqg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.444 |
| Data reduction software | HKL-2000 (2.3.10) |
| Data scaling software | HKL-2000 (2.3.10) |
| Phasing software | HKL-3000 (2.3.10) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.117 | 0.550 |
| Number of reflections | 32038 | 3211 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 97.1 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M NH4OAc, 0.05 M Sodium cacodylate pH 6.5, 30% PEG 8,000 |
