7X45

Grass carp interferon gamma related

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	291.15
Detector technology	CCD
Collection date	2021-11-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9979
Spacegroup name	P 31 1 2
Unit cell lengths	48.580, 48.580, 208.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.070 - 2.260
Rwork	0.221
R-free	0.26830
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.070	2.330
High resolution limit [Å]	2.260	2.260
Rmerge	0.112	1.146
Number of reflections	18555	16390
<I/σ(I)>	15.4
Completeness [%]	99.8
Redundancy	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.5	30% (v/v) PEG 400, 100 mM sodium cacodylate/Hydrochloric acid PH 6.5, 200 mM Lithium sulfat