7X2R
Crystal structure of the E. coli transcription termination factor Rho
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-01 |
Detector | AGILENT EOS CCD |
Wavelength(s) | 0.988 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 159.496, 101.471, 204.818 |
Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
Resolution | 19.990 - 4.400 |
R-factor | 0.2765 |
Rwork | 0.272 |
R-free | 0.31480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xav |
RMSD bond length | 0.005 |
RMSD bond angle | 1.089 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 4.530 |
High resolution limit [Å] | 4.400 | 4.400 |
Number of reflections | 170098 | 7 |
<I/σ(I)> | 0.8 | |
Completeness [%] | 86.0 | |
Redundancy | 5 | |
CC(1/2) | 0.800 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.1 | 0.4 M potassium chloride, 0.05 M magnesium chloride, 50 mM Tris pH 7.5, 13% (w/v) polyethylene glycol (PEG) 6000 |