7X2P

Legionella pneumophila effector RavL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	37.377, 54.247, 128.209
Unit cell angles	90.00, 93.61, 90.00

Refinement procedure

Resolution	33.150 - 2.890
R-factor	0.2893
Rwork	0.284
R-free	0.33680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a16
RMSD bond length	0.006
RMSD bond angle	1.062
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.150	10.000
High resolution limit [Å]	2.890	2.890
Number of reflections	9974	9974
<I/σ(I)>	0.1
Completeness [%]	85.0
Redundancy	10
CC(1/2)	0.840	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M HEPES sodium pH 7.5, 0.8M potassium tartate