7X2E

Structure of USH1C PDZ2 and coiled-coil in complex with CDHR2 C-terminal tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	54.516, 50.681, 72.847
Unit cell angles	90.00, 107.77, 90.00

Refinement procedure

Resolution	28.260 - 1.850
R-factor	0.1825
Rwork	0.181
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kbs
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.880
High resolution limit [Å]	1.850	5.010	1.850
Rmerge	0.107	0.040	0.893
Rmeas	0.117	0.044	0.968
Rpim	0.046	0.017	0.369
Total number of observations	102320
Number of reflections	16311	853	809
<I/σ(I)>	5.2
Completeness [%]	99.9	99.6	99.8
Redundancy	6.3	6	6.5
CC(1/2)		0.999	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20% w/v polyethylene glycol 3000, 100 mM tri-sodium citrate, pH 5.5.