7X0R
Crystal structure of substrate binding protein Lbp complexed wtih guanosine from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.830, 62.090, 81.300 |
| Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
| Resolution | 31.045 - 1.470 |
| R-factor | 0.1569 |
| Rwork | 0.157 |
| R-free | 0.17360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fqx |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.045 | 31.045 | 1.510 |
| High resolution limit [Å] | 1.470 | 6.570 | 1.470 |
| Rmerge | 0.046 | 0.034 | 0.373 |
| Rmeas | 0.062 | 0.048 | 0.508 |
| Total number of observations | 293817 | ||
| Number of reflections | 165622 | 1835 | 11830 |
| <I/σ(I)> | 9.73 | 20.61 | 2.15 |
| Completeness [%] | 94.4 | 92.9 | 91.3 |
| Redundancy | 1.774 | 1.782 | 1.615 |
| CC(1/2) | 0.996 | 0.994 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M zinc acetate, 16% w/v polyethylene glycol 3350 |
