7X0N
Crystal structure of sugar binding protein CbpB complexed wtih laminaribiose from Clostridium thermocellum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.750, 46.440, 116.490 |
Unit cell angles | 90.00, 106.58, 90.00 |
Refinement procedure
Resolution | 25.707 - 1.680 |
R-factor | 0.1743 |
Rwork | 0.173 |
R-free | 0.20990 |
Structure solution method | SAD |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.707 | 25.707 | 1.720 |
High resolution limit [Å] | 1.680 | 7.510 | 1.680 |
Rmerge | 0.046 | 0.023 | 0.426 |
Rmeas | 0.064 | 0.032 | 0.593 |
Total number of observations | 278714 | ||
Number of reflections | 148751 | 1588 | 11347 |
<I/σ(I)> | 11.25 | 26.08 | 2.43 |
Completeness [%] | 89.2 | 85 | 91.3 |
Redundancy | 1.874 | 1.831 | 1.875 |
CC(1/2) | 0.997 | 0.997 | 0.665 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2% Tacsimate pH 4.0, 0.1 M sodium acetate trihydrate pH 4.6, 16% w/v Polyethylene glycol 3350 |