7WXX
Crystal structure of human MMP-7 in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 77.360, 77.360, 62.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.700 - 1.500 |
| R-factor | 0.17191 |
| Rwork | 0.170 |
| R-free | 0.20343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y6c |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.271 |
| Phasing software | MOLREP (11.6.02) |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.700 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.201 | |
| Number of reflections | 29640 | 4719 |
| <I/σ(I)> | 11.81 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.8 | 11.7 |
| CC(1/2) | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 20% w/v Polyethylene glycol 6000, 100mM MES pH6.0, 200mM Lithium chloride |
