7WXS

Lysozyme protected by polyacrylamide gel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	95
Detector technology	CCD
Collection date	2021-06-18
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 43 21 2
Unit cell lengths	79.114, 79.114, 37.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.020 - 1.250
R-factor	0.1788
Rwork	0.178
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lt5
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.020	1.700	1.290
High resolution limit [Å]	1.250	1.570	1.250
Rmerge	0.119	0.186	0.571
Rmeas	0.130	0.203	0.623
Number of reflections	32552	3245	3112
<I/σ(I)>	23.4	15.5	3.6
Completeness [%]	98.3	98.3	98.3
Redundancy	6	6	6
CC(1/2)	0.994	0.970	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	50mM sodium acetate, 1.5M NaCl, pH 4.5