7WXM

Crystal Structure of PL-5 family polysaccharide lyase PanPL from Pandoraea apista at pH6.5 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07234
Spacegroup name	P 21 21 21
Unit cell lengths	36.106, 84.624, 91.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.090 - 1.230
R-factor	0.1705
Rwork	0.170
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wxj
RMSD bond length	0.009
RMSD bond angle	1.063
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.090	1.256
High resolution limit [Å]	1.230	1.230
Rmerge	0.054	0.658
Rpim	0.016	0.324
Number of reflections	78113	2704
<I/σ(I)>	21.9	2.1
Completeness [%]	96.1	67.8
Redundancy	11.3	4.9
CC(1/2)	1.000	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295.15	0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350