7WWP
Crystal structure of human Npl4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 136.325, 136.325, 126.875 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.440 - 2.990 |
| R-factor | 0.2339 |
| Rwork | 0.231 |
| R-free | 0.29050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wwq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.990 | 3.000 |
| Number of reflections | 14503 | 708 |
| <I/σ(I)> | 22.5 | 1.7 |
| Completeness [%] | 99.0 | |
| Redundancy | 27.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M HEPES (pH6.5), 16 % (w/v) PEG 3350, and 5 % (v/v) MPD |
