7WWO

Dimer form of hypothetical protein TTHA1873 from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.0723
Spacegroup name	P 61 2 2
Unit cell lengths	90.422, 90.422, 141.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.630 - 2.170
R-factor	0.1874
Rwork	0.186
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wrk
RMSD bond length	0.011
RMSD bond angle	1.590
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.630	2.240
High resolution limit [Å]	2.170	2.170
Number of reflections	18800	1575
<I/σ(I)>	5.6
Completeness [%]	99.9
Redundancy	9.9
CC(1/2)	0.959	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	60% v/v Tacsimate pH 7.0