7WW8
Crystal structure of MutT-8-oxo-dGTP complex: Reaction for 5 hr in 5 mM Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.264, 56.051, 59.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.690 - 1.640 |
R-factor | 0.1572 |
Rwork | 0.156 |
R-free | 0.18120 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3a6t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.258 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.690 | 1.700 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.062 | 0.220 |
Number of reflections | 16066 | 1534 |
<I/σ(I)> | 41.8 | |
Completeness [%] | 99.6 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | potassium sodium tartrate, sodium citrate, ammonium sulfate |