7WW4

Crystal structure of multidomain beta-1,3(4)-glucanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-10
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	120.940, 120.940, 70.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.527 - 2.230
R-factor	0.249873564816
Rwork	0.248
R-free	0.28521
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.466
Data scaling software	HKL-3000
Refinement software	PHENIX (dev_2474)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.530	2.310
High resolution limit [Å]	2.230	2.230
Rmeas	0.136
Number of reflections	28796	2685
<I/σ(I)>	17.7
Completeness [%]	98.0
Redundancy	7.2
CC(1/2)		0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.056M sodium phosphate monobasic monohydrate, 1.344M potassium phosphate dibasic