7WVT

Crystal structure of Saccharomyces cerevisiae Sfh2 complexed with phosphatidylinositol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	110
Detector technology	CCD
Collection date	2021-07-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97950
Spacegroup name	P 21 21 2
Unit cell lengths	74.541, 117.132, 42.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.770 - 1.500
R-factor	0.1702
Rwork	0.169
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.967
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.084	0.284
Number of reflections	59663	2577
<I/σ(I)>	40.1	5.6
Completeness [%]	98.0	85.6
Redundancy	4.8	2.4
CC(1/2)	0.996	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1 M MES pH 6.0, 30% PEG 1000