7WVL
Structure of P4A2 Fab in complex with Spike-RBD from SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.309, 85.309, 205.193 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.880 - 3.000 |
| R-factor | 0.236 |
| Rwork | 0.233 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7b3o |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.677 |
| Data reduction software | iMOSFLM (7.3.0) |
| Data scaling software | Aimless (7.1.012) |
| Phasing software | PHASER (7.1.012) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.880 | 3.240 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.093 | 0.743 |
| Number of reflections | 18080 | 3650 |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 9.1 |
| CC(1/2) | 0.975 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20% Peg 5K MME, 0.2 M Magnesium formate |
