7WUC

Room-temperature structure of lysozyme by serial femtosecond crystallography (BITS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	PAL-XFEL BEAMLINE NCI
Synchrotron site	PAL-XFEL
Beamline	NCI
Temperature [K]	298.15
Detector technology	CCD
Collection date	2021-12-23
Detector	RAYONIX MX225-HS
Wavelength(s)	1.30
Spacegroup name	P 43 21 2
Unit cell lengths	79.480, 79.480, 38.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.740 - 2.100
R-factor	0.2228
Rwork	0.220
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wkr
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	7605	724
<I/σ(I)>	7.38
Completeness [%]	100.0
Redundancy	590.7
CC(1/2)	0.987	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		298.5	Na-acetate, NaCl, PEG 8000