7WU8

Crystal structure of Harmonin-homology domain 2 (HHD2) of human RTEL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	65.640, 60.960, 79.710
Unit cell angles	90.00, 95.64, 90.00

Refinement procedure

Resolution	48.156 - 1.599
Rwork	0.176
R-free	0.20120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q9NZ71-F1
RMSD bond length	0.015
RMSD bond angle	1.895
Data reduction software	XDS (February 5, 2021)
Data scaling software	XDS (February 5, 2021)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.336	4.780	1.600
High resolution limit [Å]	1.599	3.390	1.599
Rmeas	0.053	0.030	0.640
Number of reflections	80150	3147	12698
<I/σ(I)>	13.48	40.78	1.73
Completeness [%]	96.8		95.3
Redundancy	3.47
CC(1/2)	0.999	0.999	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	20mM Tris pH7.4, 250mM NaCl, 5% Glycerol