7WRT

X-ray structure ofThermus thermophilus HB8 transketorase demonstrate in complex with TPP and D-erythrose-4-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	72.680, 88.860, 117.370
Unit cell angles	72.31, 88.70, 73.46

Refinement procedure

Resolution	41.860 - 2.250
Rwork	0.202
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e6k
RMSD bond length	0.009
RMSD bond angle	1.642
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.860	2.310
High resolution limit [Å]	2.250	2.250
Number of reflections	124659	9119
<I/σ(I)>	18.6
Completeness [%]	98.1
Redundancy	3.5
CC(1/2)	0.998	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100mM imidazole/MES buffer pH 6.5, 20%(v/v) ethylene glycol, 10% (w/v) PEG 8000, 24mM of D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, and N-acetyl-D-glucosamine