7WRK
Structure of hypothetical protein TTHA1873 from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-06-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.180, 42.180, 156.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.770 - 1.780 |
| R-factor | 0.1642 |
| Rwork | 0.162 |
| R-free | 0.19850 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.827 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.300 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 14566 | 804 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.8 | |
| CC(1/2) | 0.995 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 0.1 M Tris pH 8.5 and 3.0 M Sodium chloride |
