7WR2

Cryatal structure of OspC3 C-terminal ankyrin-repeat domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-05-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	39.215, 53.943, 59.932
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.090 - 1.540
R-factor	0.1705
Rwork	0.168
R-free	0.19030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wr1
RMSD bond length	0.006
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.100	1.580
High resolution limit [Å]	1.540	1.540
Rmerge	0.045	0.158
Rmeas	0.049	0.176
Number of reflections	19087	1304
<I/σ(I)>	22.58	7.87
Completeness [%]	98.6	92.6
Redundancy	6.02
CC(1/2)	0.999	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG monomethyl ether 550, 0.1 M Bis-Tris propane pH 9.0