7WPK
Methionyl-tRNA synthetase from Staphylococcus aureus complexed with L-Met
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-15 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.256, 72.773, 119.066 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.090 - 2.800 |
| R-factor | 0.2318 |
| Rwork | 0.229 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qre |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.118 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.100 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.130 |
| Number of reflections | 13216 | 1327 |
| <I/σ(I)> | 17.3 | 7 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | Sodium cacodylate, PEG 3350 |
