7WPH

SARS-CoV2 RBD bound to Fab06

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9464
Spacegroup name	P 21 21 2
Unit cell lengths	50.170, 266.350, 112.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.150 - 2.890
R-factor	0.2306
Rwork	0.229
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c01
RMSD bond length	0.012
RMSD bond angle	1.553
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.993
High resolution limit [Å]	2.890	2.890
Rmeas	0.332
Rpim	0.101
Number of reflections	34904	3389
<I/σ(I)>	8.31	1.22
Completeness [%]	99.8	96.7
Redundancy	10.4
CC(1/2)	0.994	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M magnesium formate dihydrate, 20% w/v PEG 3350