7WOL
Crystal structure of lipase TrLipB from Thermomocrobium roseum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.402, 129.402, 122.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.750 - 2.000 |
| Rwork | 0.157 |
| R-free | 0.18620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lzk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.821 |
| Number of reflections | 70819 | 4504 |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1M Succinic acid,0.1M HEPES,7,1% w/vPEG 2000 MME |
