7WNJ

Crystal structure and Cap Binding analysis of the methyltransferase of Langat virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.978910
Spacegroup name	P 2 21 21
Unit cell lengths	35.722, 91.554, 98.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.780 - 1.700
R-factor	0.1817
Rwork	0.181
R-free	0.19860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oxt
RMSD bond length	0.006
RMSD bond angle	0.737
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.780	1.763
High resolution limit [Å]	1.700	1.702
Number of reflections	35880	3143
<I/σ(I)>	25.89
Completeness [%]	98.8
Redundancy	12.31
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20% glycerol; 0.1 M Tris, pH 8.0; and 20% PEG 3350